A novel approach for increasing transformation efficiency in E. coli DH5α cells using silver nanoparticles
نویسندگان
چکیده
منابع مشابه
Effects of Temperature-Induced Changes in Membrane Composition on Transformation Efficiency in E. coli DH5α
One model of transformation proposed that heat shock destabilizes the cell membrane and allows for plasmid uptake. As lipid composition might also affect the stability of the cell membrane, we hypothesized that a lower growth temperature would alter membrane lipid composition so as to increase transformation efficiency after heat shock. Cells were grown at 20°C and 37°C and treated with calcium...
متن کاملInfluences of growth temperature and preparation of competent cells on efficiency of chemically-induced transformation in Escherichia coli DH5α
It is well known that cellular cultures grown at different temperatures will display altered membrane lipid composition to maintain a relatively constant membrane fluidity across a range of temperatures. One of the proposed theories on the mechanism of chemically-induced transformation is that a higher temperature of the heat-shock step increases the fluidity of the host cell membrane, allowing...
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a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224
M.Raffi1)†, F.Hussain, T.M.Bhatti, J.I.Akhter, A.Hameed and M.M.Hasan 1) Department of Chemical and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad-45650, Pakistan 2) Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan 3) Central Analytical Facility Division, Pakistan Institute of Nuclear Scien...
متن کاملNovel kanamycin/neomycin phosphotransferase cassette increases transformation efficiency in E. coli.
Stable transformation depends on the efficient delivery of DNA into cells and the robust expression of genes that encode proteins which provide resistance to selective (cytotoxic) compounds. We have examined the possibility that altering the 5'untranslated region (UTR) of a selectable marker may increase transformation efficiency. A 15-nucleotide synthetic UTR (the so-called universal translati...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2019
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-019-1640-9